Review

cell culture murine c2c12 skeletal muscle cell line (ATCC)

Name: cell culture murine c2c12 skeletal muscle cell line
Brand: ATCC
Rating: 99 (9158 reviews)

ATCC is a verified supplier

ATCC manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

ATCC cell culture murine c2c12 skeletal muscle cell line

SCE changes cell morphology during myogenic differentiation. <t>C2C12</t> myoblasts were cultured in growth medium until 80% confluence and then changed to DMEM containing 2% horse serum and 1, 5 and 10 ng/ml SCE for 5 days. The medium was changed every 2 days and fresh SCE was added. (A) Cell viability was assessed using the XTT assay (n=6 per group). (B) Images of morphological changes were obtained using light microscopy at the end of the experiment (5 days) (n=4 per group). Bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Cell Culture Murine C2c12 Skeletal Muscle Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell culture murine c2c12 skeletal muscle cell line/product/ATCC
Average 99 stars, based on 9158 article reviews

cell culture murine c2c12 skeletal muscle cell line - by Bioz Stars, 2026-02

99/100 stars

Images

1) Product Images from "Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro"

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

Journal: Molecular Medicine Reports

doi: 10.3892/mmr.2024.13250

SCE changes cell morphology during myogenic differentiation. C2C12 myoblasts were cultured in growth medium until 80% confluence and then changed to DMEM containing 2% horse serum and 1, 5 and 10 ng/ml SCE for 5 days. The medium was changed every 2 days and fresh SCE was added. (A) Cell viability was assessed using the XTT assay (n=6 per group). (B) Images of morphological changes were obtained using light microscopy at the end of the experiment (5 days) (n=4 per group). Bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Figure Legend Snippet: SCE changes cell morphology during myogenic differentiation. C2C12 myoblasts were cultured in growth medium until 80% confluence and then changed to DMEM containing 2% horse serum and 1, 5 and 10 ng/ml SCE for 5 days. The medium was changed every 2 days and fresh SCE was added. (A) Cell viability was assessed using the XTT assay (n=6 per group). (B) Images of morphological changes were obtained using light microscopy at the end of the experiment (5 days) (n=4 per group). Bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Techniques Used: Cell Culture, XTT Assay, Light Microscopy

SCE promotes myotube formation by C2C12 cells. C2C12 myoblasts were cultured in a DMEM containing 2% horse serum and SCE (0,1, 5 and 10 ng/ml) for 5 days. (A) Immunofluorescence staining for the myotube marker, MyHC (green), and the nuclei marker, DAPI (blue), revealed myotube formation by C2C12 cells (n=4 per group). (B) Myotube diameters in randomly selected fields were quantified using an image analysis program. ***P<0.01 vs. the control group. Scale bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Figure Legend Snippet: SCE promotes myotube formation by C2C12 cells. C2C12 myoblasts were cultured in a DMEM containing 2% horse serum and SCE (0,1, 5 and 10 ng/ml) for 5 days. (A) Immunofluorescence staining for the myotube marker, MyHC (green), and the nuclei marker, DAPI (blue), revealed myotube formation by C2C12 cells (n=4 per group). (B) Myotube diameters in randomly selected fields were quantified using an image analysis program. ***P<0.01 vs. the control group. Scale bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Techniques Used: Cell Culture, Immunofluorescence, Staining, Marker, Control

SCE increases the expression of muscle-specific factors related to myogenesis. C2C12 myoblasts were induced to differentiate in DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml), and analyzed by (A) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). The expression of MyHC, MyoD, myogenin and Myf5 over the time course of myogenesis was detected through (C) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group) in cells treated with SCE. *P<0.05, **P<0.01 and ***P<0.001 vs. the control group; #P<0.05, ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. Non-β-catenin and β-catenin were detected by western blotting depending on (E) the concentration or (F) the time of SCE. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; MyHC, myosin heavy chain; MyoD, myogenic differentiation 1; Myf5, Myogenic Factor 5.

Figure Legend Snippet: SCE increases the expression of muscle-specific factors related to myogenesis. C2C12 myoblasts were induced to differentiate in DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml), and analyzed by (A) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). The expression of MyHC, MyoD, myogenin and Myf5 over the time course of myogenesis was detected through (C) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group) in cells treated with SCE. *P<0.05, **P<0.01 and ***P<0.001 vs. the control group; #P<0.05, ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. Non-β-catenin and β-catenin were detected by western blotting depending on (E) the concentration or (F) the time of SCE. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; MyHC, myosin heavy chain; MyoD, myogenic differentiation 1; Myf5, Myogenic Factor 5.

Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Control, Concentration Assay, Reverse Transcription, Real-time Polymerase Chain Reaction

SCE upregulates the expression of biogenesis-regulating factors during C2C12 differentiation. C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml) and analyzed using (A and E) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and subjected to (C and F) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group). GAPDH was used as a loading control. **P<0.01 and ***P<0.001 vs. the control group; ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; PGC-1a, peroxisome proliferator-activated receptor-gamma coactivator-1 α; NRF1, nuclear respiratory factor 1.

Figure Legend Snippet: SCE upregulates the expression of biogenesis-regulating factors during C2C12 differentiation. C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml) and analyzed using (A and E) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and subjected to (C and F) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group). GAPDH was used as a loading control. **P<0.01 and ***P<0.001 vs. the control group; ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; PGC-1a, peroxisome proliferator-activated receptor-gamma coactivator-1 α; NRF1, nuclear respiratory factor 1.

Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

SCE activates the AMPK-HDAC5 pathways in myotubes. (A and C) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5, 10 ng/ml) and analyzed by western blotting (upper panel). Quantification of protein expression levels (n=3 per group) (lower panel). *P<0.05 and ***P<0.01 vs. the control. (B and D) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (upper panel). Quantification of protein expression levels (n=3 per group) (down). *P<0.05 and ***P<0.01 vs. the DMSO; ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; AMPK, AMP-activated protein kinase; HDAC5, histone deacetylase 5; p-, phosphorylated.

Figure Legend Snippet: SCE activates the AMPK-HDAC5 pathways in myotubes. (A and C) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5, 10 ng/ml) and analyzed by western blotting (upper panel). Quantification of protein expression levels (n=3 per group) (lower panel). *P<0.05 and ***P<0.01 vs. the control. (B and D) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (upper panel). Quantification of protein expression levels (n=3 per group) (down). *P<0.05 and ***P<0.01 vs. the DMSO; ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; AMPK, AMP-activated protein kinase; HDAC5, histone deacetylase 5; p-, phosphorylated.

Techniques Used: Western Blot, Expressing, Control, Histone Deacetylase Assay

SCE regulates AKT/mTOR and its downstream effectors in myotubes. (A) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentration of SCE (1, 5 and 10 ng/ml) and analyzed using western blotting (left). Quantification of protein expression levels (n=3 per group) (right). **P<0.01 and ***P<0.001 vs. the control. (B) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (left). Quantification of protein expression levels (n=3 per group) (right). *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO; #P<0.05, ##P<0.01 and ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; p-, phosphorylated; p70S6K1, ribosomal protein S6 kinase B1.

Figure Legend Snippet: SCE regulates AKT/mTOR and its downstream effectors in myotubes. (A) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentration of SCE (1, 5 and 10 ng/ml) and analyzed using western blotting (left). Quantification of protein expression levels (n=3 per group) (right). **P<0.01 and ***P<0.001 vs. the control. (B) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (left). Quantification of protein expression levels (n=3 per group) (right). *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO; #P<0.05, ##P<0.01 and ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; p-, phosphorylated; p70S6K1, ribosomal protein S6 kinase B1.

Techniques Used: Concentration Assay, Western Blot, Expressing, Control

Image Search Results

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE changes cell morphology during myogenic differentiation. C2C12 myoblasts were cultured in growth medium until 80% confluence and then changed to DMEM containing 2% horse serum and 1, 5 and 10 ng/ml SCE for 5 days. The medium was changed every 2 days and fresh SCE was added. (A) Cell viability was assessed using the XTT assay (n=6 per group). (B) Images of morphological changes were obtained using light microscopy at the end of the experiment (5 days) (n=4 per group). Bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Cell Culture, XTT Assay, Light Microscopy

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE promotes myotube formation by C2C12 cells. C2C12 myoblasts were cultured in a DMEM containing 2% horse serum and SCE (0,1, 5 and 10 ng/ml) for 5 days. (A) Immunofluorescence staining for the myotube marker, MyHC (green), and the nuclei marker, DAPI (blue), revealed myotube formation by C2C12 cells (n=4 per group). (B) Myotube diameters in randomly selected fields were quantified using an image analysis program. ***P<0.01 vs. the control group. Scale bar, 50 µm. SCE, Saururus chinensis (Lour.) Baill. Extract.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Cell Culture, Immunofluorescence, Staining, Marker, Control

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE increases the expression of muscle-specific factors related to myogenesis. C2C12 myoblasts were induced to differentiate in DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml), and analyzed by (A) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). The expression of MyHC, MyoD, myogenin and Myf5 over the time course of myogenesis was detected through (C) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group) in cells treated with SCE. *P<0.05, **P<0.01 and ***P<0.001 vs. the control group; #P<0.05, ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. Non-β-catenin and β-catenin were detected by western blotting depending on (E) the concentration or (F) the time of SCE. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; MyHC, myosin heavy chain; MyoD, myogenic differentiation 1; Myf5, Myogenic Factor 5.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Concentration Assay, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE upregulates the expression of biogenesis-regulating factors during C2C12 differentiation. C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5 and 10 ng/ml) and analyzed using (A and E) RT-qPCR (n=3 per group) and (B) western blotting (n=3 per group). Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and subjected to (C and F) RT-qPCR (n=3 per group) and (D) western blotting (n=3 per group). GAPDH was used as a loading control. **P<0.01 and ***P<0.001 vs. the control group; ##P<0.01 and ###P<0.001 vs. the SCE-treated group at the corresponding indicated times. SCE, Saururus chinensis (Lour.) Baill. extract; RT-qPCR, reverse transcription-quantitative PCR; PGC-1a, peroxisome proliferator-activated receptor-gamma coactivator-1 α; NRF1, nuclear respiratory factor 1.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE activates the AMPK-HDAC5 pathways in myotubes. (A and C) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentrations of SCE (1, 5, 10 ng/ml) and analyzed by western blotting (upper panel). Quantification of protein expression levels (n=3 per group) (lower panel). *P<0.05 and ***P<0.01 vs. the control. (B and D) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (upper panel). Quantification of protein expression levels (n=3 per group) (down). *P<0.05 and ***P<0.01 vs. the DMSO; ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; AMPK, AMP-activated protein kinase; HDAC5, histone deacetylase 5; p-, phosphorylated.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Western Blot, Expressing, Control, Histone Deacetylase Assay

Journal: Molecular Medicine Reports

Article Title: Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro

doi: 10.3892/mmr.2024.13250

Figure Lengend Snippet: SCE regulates AKT/mTOR and its downstream effectors in myotubes. (A) C2C12 myoblasts were induced to differentiate in a DMEM containing 2% horse serum, treated for 5 days with different concentration of SCE (1, 5 and 10 ng/ml) and analyzed using western blotting (left). Quantification of protein expression levels (n=3 per group) (right). **P<0.01 and ***P<0.001 vs. the control. (B) Cells were treated with 10 ng/ml SCE in DM for 1, 3 and 5 days and western blotting was performed using the indicated antibodies (left). Quantification of protein expression levels (n=3 per group) (right). *P<0.05, **P<0.01 and ***P<0.001 vs. the DMSO; #P<0.05, ##P<0.01 and ###P<0.001 vs. the DMSO at the indicated time points. SCE, Saururus chinensis (Lour.) Baill. extract; p-, phosphorylated; p70S6K1, ribosomal protein S6 kinase B1.

Article Snippet: Cell culture Murine C2C12 skeletal muscle cell line (cat. no. CRL-1772) were purchased from American Type Culture Collection.

Techniques: Concentration Assay, Western Blot, Expressing, Control

Review

Structured Review

Images

1) Product Images from "Saururus chinensis (Lour.) Baill. extract promotes skeletal muscle cell differentiation by positively regulating mitochondrial biogenesis and AKT/mTOR signaling in vitro"

Similar Products

Image Search Results